Satellite Cell Proliferation And Differentiation

Expression of r3hdml increases during skeletal muscle development and differentiation. However both gain and loss of function studies demonstrated that mir 101a had no significant influence on satellite cell proliferation. Therefore our results provide a new insight on mir 101a in the regulation of goat skeletal muscle satellite cell proliferation and differentiation.

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Materials and methods 2 1.

Satellite cell proliferation and differentiation.

Expression of r3hdml increases during muscle regeneration induced by muscle injury. Recovery of handgrip strength after ctx injection was significantly impaired in r3hdml ko mice which is rescued by r3hdml. R3hdml is a satellite cell secreted protein which is required for muscular development differentiation and regeneration. Our results indicate that r3hdml is required for skeletal muscle development regeneration and in particular satellite cell proliferation and differentiation.

R3hdml is a protein secreted from satellite cells. Satellite cell activation is measured by the extent of proliferation and differentiation. Typically satellite cell content is expressed per muscle fiber or as a percentage of total nuclear content the sum of satellite cell nuclei and myonuclei. In the present study we analyzed the expression of runx1 during satellite cell differentiation and evaluated the role of runx1 in regulating the proliferation and differentiation of a myogenic line c2c12 cells.

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